Talk:20.109(F07): Luciferase assays and RNA prep

Luciferase Assay Data

Refer to table from first day of module to know what part of the Renilla luciferase gene was targeted by each group.
Please list values for each of your duplicate samples, separated by a comma.
Please calculate out any dilutions that you may have performed on your lysates so that the values listed here reflect the measurements for undiluted samples.
If there were any unusual things that are worth noting about your experiment, please note them below the table for your day of lab and "sign" your comment with your team color.

T/R lab

Sample	Green	Purple	Red	Blue	Pink	Yellow
No DNA	0.0, .083	ff: 0.008, 0.009, R: 0.053, 0.054	394.3, 470.9	ff: 0.017,0.005, R: 0.195, 0.045	ff: 0.012, 0.012; R: 0.049, 0.054	ff: 0.0103, 0.0031; R: 0.0903, 0.0616
plasmid only	11.12, 406.2	ff: 119.0, 109.3, R: 3826, 3299	5864, 9516	ff: 74.52, 66.88, R: 2198, 2009	ff: 21.42, 25.56; R: 618.0, 747.9	ff: 181.9, 195.1; R: 5175, 5496
siRNA only	0.009, .049	ff: 0.012, 0.013, R: .063, .051	354, 467.6	ff: 0.010, 0.010 R: 0.044, 0.104	ff: 0.007, 0.005; R: 0.064, 0.059	ff: 0.0068, 0.0257; R: 0.0866, 0.0721
plasmid+siRNA (validated)	23.21, 843.7	ff: 61.80, 86.20, R: 136.4, 177.7	0.058, 0.074	ff: 48.06 78.31, R: 185.7, 317. 2	ff: 29.88, 14.24; R: 81.25, 42.32	ff: 245.1, 255.8; R: 443.5, 438.5
plasmid+siRNA (scrambled)	.521, 19.31	ff: 76.87, 88.13, R: 2184, 2819	8705, 4133	ff: 86.87, 66.71 R: 2805, 2085	ff: 26.32, 15.02; R: 760.3, 447.6	ff: 229.3, 220.0; R: 6976, 7308
plasmid+siRNA (experimental)	11.14, 354.3	ff: 124.2, 124.2, R: 1170, 1209	0.113, 0.114	ff: 120.2, 74.77, R: 1881, 1327	ff: 22.33, 12.16; R: 237.1, 127.7	ff: 223.1, 233.2; R: 748.7, 717.1

W/F lab

Sample	Green	Purple	Red	Blue	Pink	Yellow
No DNA	--. 0.042	.048, .050	ff 0.059, 0.096; R 0.658, 1.164	ff 0.000,0.288 R 0.486,0.183	ff 0.006,0.011 R 0.019,0.057
plasmid only	479.6, 206.2	2132, 17.75	(1/10 dil) ff 9.332, 78.35; R 252.1, 1773	ff 53.18,60.96 R 138.1,66.05	ff 2.276,40.13 R 10.41,1317
siRNA only	0.055, 0.058	.056, .013	ff 0.080, 0.120; R 0.481, 0.575	ff 0.047,0.117 R 0.241,0.299	ff 0.011,0.012 R 0.024,0.140
plasmid+siRNA (validated)	210.6, 130.5	158.3, 0.963	ff 781.6, 731.0; R 2114, 2537	ff 57.02,57.56 R 28.75,13.65	ff 5.476,5.378 R 12.73,28.36
plasmid+siRNA (scrambled)	1749, 1478	1167, 3.408	(1/10 dil) ff 162.2, 85.75; R 5424, 2225	ff 58.33,59.67 R 149.8,42.17	ff 6.211,55.10 R 48.15,2427
plasmid+siRNA (experimental)	113, 107.1	202.9, 0.779	ff 926.4, 644.1; R 2925, 1880	ff 69.26,63.04 R 45.68,57.43	ff 4.872,47.39 R 3.998,210.4