Talk:20.109(F07):Start-up genome engineering
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| oligo name | oligo number | oligo sequence | primer record | enzymes added with insert | |
|---|---|---|---|---|---|
| TeacherOligo_top | NO196 | 5' GAT CTG CGC ACG CGT | teacher sample oligo record | FspI and MluI | |
| TeacherOligo_btm | NO197 | 5'G,ATC,ACG,CGT,GCG,CA | |||
| M13KO7seq_2101_fwd | NO198 | 5'- CAA GGC ACT GAC CCC GTT AAA ACT TAT TAC -3' | |||
| M13KO7seq_2151_fwd | NO199 | 5'- AAA AGC CAT GTA TGA CGC TTA CTG GAA CGG -3' |
T/R lab
| oligo name | oligo number | oligo sequence | primer record | enzymes added with insert |
|---|---|---|---|---|
| TRpink_top | NO202 | 5’ GAT CTC GAC GGC GCC 3’ | Oligo design record | Sal I |
| TRpink_btm | NO203 | 5’ G ATC GGC GCC GTC GA 3’ | Oligo design record | Sfo I |
| TRyellow_top | NO204 | 5' GAT CTA GAT ATC 3' edit:5'gat cta gaC GAt atc |
Yellow Insert Design | Xba I, Eco RV |
| TRyellow_btm | NO205 | 5' G ATC GAT ATC TA 3' edit: 5'G atc gat atc gtc ta |
Yellow Insert edits! | |
| TRblue_top | NO206 | 5' GAT CAG ATC TTA GGC GCC CGA 3' | Blue Insert Design | BglII |
| TRblue_btm | NO207 | 5' G ATC TGC GGC GCC TAA GAT CT 3' | Blue Insert Design | SfoI |
| TRpurple_top | NO208 | 5' GAT CTG ATC AGT ACT 3' | Purple Insert Design | BclI, ScaI |
| TRpurple_btm | NO209 | 5' GAT CAG TAC TGA TCA 3' | ||
| TRred_top | NO210 | 5' GAT CTT AAG GAC GTC | Red Insert Design | AflII ZraI |
| TRred_btm | NO211 | 5' G ATC GAC GTC CTT AA | ||
| TRgreen_top | NO212 | 5' GAT CGG TAC CAC GTC 3' | Green Insert Design | KpnI BmgI |
| TRgreen_btm | NO213 | 5' G ATC GAC GTG GTA CC 3' |
W/F lab
| oligo name | oligo number | oligo sequence | primer record | enzymes added with insert | ||
|---|---|---|---|---|---|---|
| WFpink_top | NO214 | G A T , C A T , G A A , T T C, G A T , A T C | pink insert design | EcoRI, EcoRV | ||
| WFpink_btm | NO215 | G A T , C G A , T A T , C G A , A T T , C A T | ||||
| WFyellow_top | NO | |||||
| WFyellow_btm | NO | |||||
| WFblue_top | NO216 | gatcagatctcccacgtc | Blue Insert Design | BglII,BmgBI | ||
| WFblue_btm | NO217 | gatcgacgtgggagatct | ||||
| WFpurple_top | NO218 | gat/cGA/ATT/CAC/GTC | Purple Insert Design | EcoRI, BmgBI | ||
| WFpurple_btm | NO219 | g/atc/GAC/GTG/AAT/TC | ||||
| WFred_top | NO220 | gat/cGA/ATT/CTT/GTT/AAC | Red Insert Design | EcoRI, HpaI | ||
| WFred_btm | NO221 | g/atc/GTT/AAC/AAG/AAT/TC | ||||
| WFgreen_top | (NO218) | 5’-gat,cga,att,cac,gtc,gat,c-3’ | Green Insert Design | EcoRI, BmgBI | ||
| WFgreen_btm | (NO219) | 5’-g,atc,gac,gtg,aat,tcg,atc-3’ |
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