TFB II
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| Reagent | F.W. | 100 ml |
|---|---|---|
| 10 mM MOPS | 209.3 | 0.209 |
| 75 mM CaCl2 | 147 | 1.102 |
| 10 mM RbCl | 120.9 | 0.121 |
| 15% Glycerol | N/A | 15 ml |
pH 6.5 w/ KOH. Filter sterilize.
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