TFB I

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DH5α Competent Cells

TFB I
Reagent F.W. 200 ml 400 ml 800 ml
30 mM Potassium acetate 98.14 0.59 g 1.18 g 2.36 g
100mM RbCl 120.9 2.42 g 4.84 g 9.68 g
10 mM CaCl2 147 0.294 g 0.59 g 1.18 g
50 mM MnCl2 197.9 1.98 g 3.96 g 7.92 g
15% Glycerol N/A 30 mL 60 mL 120 mL

pH 5.8 w/ Acetic Acid (do not overshoot—correcting with KOH will cause irreversible precipitation). Filter sterilize.