Synthetic complete (SC) medium

Dropout mix

• A dry powder mix of all amino acids and nucleic acids.
• Omit the components used for selection
• Mix end over end with clean glass marbles 15 minutes.

• Adenine 0.5 g
• Alanine 2.0 g
• Arginine 2.0 g
• Asparagine 2.0 g
• Aspartic acid 2.0 g
• Cysteine 2.0 g
• Glutamine 2.0 g
• Glutamic acid 2.0 g
• Glycine 2.0 g
• Histidine 2.0 g
• Inositol 2.0 g
• Isoleucine 2.0 g
• Leucine 10.0 g
• Lysine 2.0 g
• Methionine 2.0 g
• p-aminobenzoic acid 2.0 g
• Phenylalanine 2.0 g
• Proline 2.0 g
• Serine 2.0 g
• Threonine 2.0 g
• Tryptophan 2.0 g
• Tyrosine 2.0 g
• Uracil 2.0 g
• Valine 2.0 g

Total weight 54 g

For SC -ura -trp dropout medium additions to SORB plates

• Total weight of mix is 50 g
• For SORB plates with 0.6 g/liter SC dropout medium addition
  • Add 24 mg/liter of Uracil or 24 ml/liter of an 0.1% solution
  • Add 24 mg/liter of Tryptophan or 2.4 ml/liter of a 1% solution

Final SC medium, per liter, autoclave

• Bacto-yeast nitrogen base w/o amino acids (0.67%) 6.7 g
• Glucose (2%) 20 g
• Dropout mix 2.0 g
• Bacto Agar (2%) 20 g

• Add 80 mg/liter of Uracil or 80 ml/liter of an 0.1% solution
• Add 80 mg/liter of Tryptophan or 8 ml/liter of a 1% solution