SynBERC:Seminar series/Ichiro Matsumura

From OpenWetWare
Jump to navigationJump to search

Acinetobacter baylyi ADP1 as a chassis for the directed evolution of genes and genomes
Ichiro Matsumura, Associate Professor of Biochemistry, Emory University School of Medicine Follow link to see video: Wedesday, April 22, 2009
noon-1 pm ET
RM 449 (Kiva/Patil,) Stata Center (Building 32,) MIT

Synthetic biologists generally build upon the Escherichia coli chassis, which usually necessitates a life of cloning. Acinetobacter baylyi sp. ADP1 offers two significant advantages over E. coli: natural competence, and efficient homologous recombination. I will explain how these natural advantages obviate cloning and streamline the directed evolution of genes and genomes. I will also describe the engineering of a broad host range expression vector pBAV1K (Biobrick Accepting Vector) that replicates and produces proteins in a wide variety of gram negative and gram positive bacteria. These tools enable the facile laboratory evolution of genes and chromosomes, without restriction digests or ligation reactions.