Suh: lab responsibilities

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DNA Gels & Imager

Inventory Check Item Minimum Level Agarose Stock Gel Loading Dye Stock Gel Loading Dye Aliquot Ethidium Bromide Stock Ethidium Bromide Aliquot Invitrogen 1kb Plus Ladder stock Invitrogen 1kb Plus Ladder Aliquot Invitrogen 10 bp Ladder stock Invitrogen 10 bp Ladder aliquot Printer Film

Maintenance Check Item Task Gel Imager Wipe Down light box w/ 70% EtOH, inspect for cuts Electrophoresis Units Inspect for damage/missing parts EtBr Waste Send for Haz Waste Pickup, place new container


Protein Gels

Inventory Check Item Minimum Level 7% Tris-Acetate Gel Cassettes 4-12% Bis-Tris Gel Cassettes Tris-Acetate Running buffer SDS-MOPS Running Buffer SDS-MES Running Buffer Transfer Buffer Milk Powder LDS Sample Buffer Bovine Serum Albumin Rainbow Protein Marker Film Nitrocellulose Whatman Paper Plastic Sleeves Antibody Stocks Antibody Aliquots Blot Pads Spatulas

Maintenance Check Item Task Electrophoretic Units Inspect for damage, missing parts