Streptomycin

Mode of Action

Inhibits protein synthesis; binds to 30S ribosomal subunit.

The rpsL50 gene mutation conveys streptomycin resistance. Resistance is recessive, so the presence of a wild type rpsL gene will convey sensitivity. This allows counter-selection against the presence of the wt rpsL gene. Mutations of wild type genes to convey resistance are common.

Usage Notes

1. 100 mg/mL stock solution in H₂O.
   It is barely soluble in ethanol so you must dissolve it in H₂O.
2. Filter-sterilize with 0.22 μm filter.
3. Add 1 mL per liter of culture.

References

J. H. Miller. A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria. Cold Spring Harbor Laboratory Press, 1992.

J. Sambrook and D. W. Russell. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, 3rd edition, 2001.