Springer Lab:Protocols

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Protocols

S. cerevisiae Protocols

• Yeast Nomenclature
• Yeast Transformation (Gietz) - Our standard yeast transformation protocol.
• Transformation Yeast 96 well
• Quick and easy isolation of genomic DNA from Yeast
• Sporulation of diploid yeast
• Random Spore Analysis

Recipes

Growth media

• YNB media

Antibiotics

• Antibiotics

High Throughput Techniques

• Tips on handling plates

Isothermal Assembly

• Isothermal Assembly
• Isothermal Kits

Flow Cytometry

• FCS file format

Lab Maintenance

• Cleaning Glass Beads