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Nikon Ti microscope)

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Configuration

General

• find out more about the Nikon Ti on the official Nikon website (http://www.nikoninstruments.com/ti/)
• the Nikon Ti is equipped with a perfect focus unit (http://www.microscopyu.com/articles/livecellimaging/perfectfocus.html)
• the microscope is controlled by Micro-Manager (http://micro-manager.org/)
• control via Matlab is also possible (http://valelab.ucsf.edu/~MM/MMwiki/index.php/Matlab)

Objectives

Coming soon...

Cameras

• Scion CFW-1612M (Bf/Phase camera, E100 port, 12-bit, dimension: 1600 x 1200, pixel size: 4.40 um x 4.40 um)
  • see http://www.scioncorp.com/pages/CFW1612M.htm

• Hamamatsu Orca R2 (Bf/Fluorescence camera, R100 port, 12- and 16-bit, dimension: 1344 x 1024, pixel size: 6.45 um x 6.45 um)
  • see http://hamamatsucameras.com/orca-r2/

Filter cubes

• 1. DAPI (Semrock LF405-A: excitation 390/40, dichroic 405, emission 452/45)
• 2. CFP (excitation 438/24, dichroic 458, emission 483/32)
• 3. GFP (excitation 472/30, dichroic 495, emission 520/35)
• 4. YFP (excitation 500/24, dichroic 520, emission 542/27)
• 5. RFP (excitation 562/40, dichroic 593, emission 641/75), this filter was designed for true RFPs like mCherry
• 6. Bf/Phase

filters for the lasers

Coming soon....

Maintenance log

• Install new DAPI filter set. A new cfg file was genereated for micromanager.
• 2011.05.06 Install new versions of micromanager 1.3.46, 1.3.41
• 2011.05.06 Changed drivers versions from

Ti Control: Ver 1.0.54
Ti SDK: Ver 1.0.78
Eclipse Ti: Ver.V.2.10

To-do list:

• Matlab problems with PSF. Cannot detect the state of the PSF.
  
• The PSF does not engage when moving in several positions of the plate.