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TOP10 chemically competent cells: Difference between revisions
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TOP10 chemically competent cells (view source)
Revision as of 14:09, 2 February 2007
, 2 February 2007→Preparing competent cells
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* Test competence (see below) | * Test competence (see below) | ||
** Good cells should yield around 100 - 400 colonies | ** Good cells should yield around 100 - 400 colonies | ||
* Thawing and refreezing partially used cell aliquots dramatically reduces transformation efficiency by about 3x the first time, and about 6x total after several freeze/thaw cycles. | |||
===CCMB80 buffer=== | ===CCMB80 buffer=== | ||
