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McClean: Sequencing Colony PCR Product: Difference between revisions
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McClean: Sequencing Colony PCR Product (view source)
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## Bring the total volume to 20μL with DI water. For example, if your DNA + primer volume is 3.3μL, then insert 16.7μL of water. | ## Bring the total volume to 20μL with DI water. For example, if your DNA + primer volume is 3.3μL, then insert 16.7μL of water. | ||
### Some of the webpages say the total volume should be 24μL. In person I was told 20μL. When I asked about the contradiction, they said 20μL is good but 24μL is also good... >_< | ### Some of the webpages say the total volume should be 24μL. In person I was told 20μL. When I asked about the contradiction, they said 20μL is good but 24μL is also good... >_< | ||
## Label the strip tubes individually. Then wrap them in foil and on a | ## Label the strip tubes individually. Then wrap them in foil and on a piece of tape on the foil write your PI's name, your name, the date, and the name of the set of samples. (The foil itself isn't special, a bag could be used too, anything to keep them together and labeled.) | ||
## Go to the Biotech facility (room 2360 at the time of writing this) and log in to the computer. If the tubes are individual and not connected select Discount Sequencing for $10/sample. Discount Sequencing-Striptube, $7.50/sample, is for connected 0.2ul PCR tubes. Place the printed order on the marked plastic shelf and leave your | ## Go to the Biotech facility (room 2360 at the time of writing this) and log in to the computer. If the tubes are individual and not connected select Discount Sequencing for $10/sample. Discount Sequencing-Striptube, $7.50/sample, is for connected 0.2ul PCR tubes. Place the printed order on the marked plastic shelf and leave your labeled sample in the fridge. | ||
## If you are still confused, the people at the facility are extremely patient and helpful. Talking to them in person may be best. | ## If you are still confused, the people at the facility are extremely patient and helpful. Talking to them in person may be best. | ||
