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IGEM:Hong Kong HKUST/Investigations/ Plasmid Cloning of BFP Generator Using Standard Assembly: Difference between revisions
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IGEM:Hong Kong HKUST/Investigations/ Plasmid Cloning of BFP Generator Using Standard Assembly (view source)
Revision as of 06:28, 5 June 2016
, 5 June 2016→Gel Electrophoresis, Purification, and Nanodrop Test
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===Gel Electrophoresis, Purification, and Nanodrop Test=== | ===Gel Electrophoresis, Purification, and Nanodrop Test=== | ||
Gel Electrophoresis was performed for 30 minutes using 1% agarose gel and midori green as dye. The voltage and current used were 130 V and 400 mA respectively. The expected bands were | Gel Electrophoresis was performed for 30 minutes using 1% agarose gel and midori green as dye. The voltage and current used were 130 V and 400 mA respectively. The expected bands were 2726 bp for J61002-BBa_J23110 and 771 bp for BBa_K592023. After the gel photo was obtained, desired band was taken and purified according to the protocol. The purified band then tested for its concentration and protein and salt contamination by using Nanodrop machine. | ||
===Ligation=== | ===Ligation=== | ||
