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IGEM:Hong Kong HKUST/Investigations/ Plasmid Cloning of BFP Generator Using Standard Assembly: Difference between revisions
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IGEM:Hong Kong HKUST/Investigations/ Plasmid Cloning of BFP Generator Using Standard Assembly (view source)
Revision as of 01:57, 5 June 2016
, 5 June 2016→Introduction
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==Introduction== | ==Introduction== | ||
By using BioBricks Standard Assembly, we can assemble two BioBricks containing our desired parts into the same plasmid, this can then be transformed into a cell and cloned to obtain our desired results. The objective of our project was to create a recombinant cell containing a BFP generator from the BioBricks BBa_J23110 and BBa_K592023. BBa_J23110 is a constitutive promoter, and BBa_K592023 is an intermediate containing a BBa_K592100 Blue Fluorescent Protein and a BBa_B0032 RBS. After combining the two BioBricks by restriction digestion and ligation, then transforming the recombinant plasmid into the cell, blue fluorescent protein producing E. Coli could be obtained. | By using BioBricks Standard Assembly, we can assemble two BioBricks containing our desired parts into the same plasmid, this can then be transformed into a cell and cloned to obtain our desired results. The objective of our project was to create a recombinant cell containing a BFP generator from the BioBricks BBa_J23110 and BBa_K592023. BBa_J23110 is a constitutive promoter, and BBa_K592023 is an intermediate containing a BBa_K592100 Blue Fluorescent Protein and a BBa_B0032 RBS. After combining the two BioBricks by restriction digestion and ligation, then transforming the recombinant plasmid into the cell, blue fluorescent protein producing ''E. Coli'' could be obtained. | ||
The details of the BioBricks used are shown in Table 1. BioBricks used in this investigation. | The details of the BioBricks used are shown in Table 1. BioBricks used in this investigation. | ||
