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CHEM-ENG590E Wiki Textbook:Microcontact Printing: Difference between revisions
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CHEM-ENG590E Wiki Textbook:Microcontact Printing (view source)
Revision as of 14:58, 14 April 2016
, 14 April 2016→Applications
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'''Biomaterial Patterning<sup>[5]</sup>''' | '''Biomaterial Patterning<sup>[5]</sup>''' | ||
[[Image:F5.medium.gif | [[Image:F5.medium.gif|right|thumbnail|'''Figure X''' Schematic of method for generating micropatterned cocultures, from Bhatia et al.<sup>[5]</sup> (A) Borosilicate substrates were coated with photoresist (a UV-sensitive polymer) and exposed to light through a mask, creating a photoresist pattern. (B) Photoresist was visualized using epifluorescent microscopy. (C) Collagen I was immobilized, followed by removal of the photoresist, which yielded a collagen-glass pattern. (D) Indirect immunofluorescence verified collagen immobilization. (E) Patterned substrates were exposed to hepatocytes, resulting in micropatterned hepatocytes. (F) 200 μM lanes of hepatocytes with 500 μM lane spacing. (G) Addition of fibroblasts to create micropatterned cocultures. (H) 2 distinct cell types in micropatterned coculture are clear.]] | ||
