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TOP10 chemically competent cells: Difference between revisions
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TOP10 chemically competent cells (view source)
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This protocol is a variant of the Hanahan protocol <cite>Hanahan91</cite> using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the [[Media:pat6855494.pdf | Bloom05 patent]] as well. This protocol has been tested on TOP10 and | This protocol is a variant of the Hanahan protocol <cite>Hanahan91</cite> using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the [[Media:pat6855494.pdf | Bloom05 patent]] as well. This protocol has been tested on TOP10, MachI and [[Talk:TOP10 chemically competent cells|BL21(DE3)]] cells. See [[Bacterial Transformation]] for a more general discussion of other techniques. The [[Media:pat6960464.pdf | Jesse '464 patent]] describes using this buffer for DH5α cells. The [[Media:pat6709852.pdf | Bloom04]] patent describes the use of essentially the same protocol for the Invitrogen Mach 1 cells. | ||
===Preparing glassware and media=== | ===Preparing glassware and media=== | ||
