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Etchevers:Extraction ARN francais: Difference between revisions
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Etchevers:Extraction ARN francais (view source)
Revision as of 08:04, 14 December 2006
, 14 December 2006→Materiels
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==Materiels== | ==Materiels== | ||
Qiagen [http://http://www1.qiagen.com/literature/handbooks/PDF/RNAStabilizationAndPurification/FromAnimalAndPlantTissuesBacteriaYeastAndFungi/RNY_Mini/1035969_HB_BenchProtocol.pdf RNeasy Mini extraction kit] (ref 74104) or Midi (75144) | *Qiagen [http://http://www1.qiagen.com/literature/handbooks/PDF/RNAStabilizationAndPurification/FromAnimalAndPlantTissuesBacteriaYeastAndFungi/RNY_Mini/1035969_HB_BenchProtocol.pdf RNeasy Mini extraction kit] (ref 74104) or Midi (75144) | ||
ethanol 70% et 100% | *ethanol 70% et 100% | ||
azote liquide | *azote liquide | ||
mortier et pilon en agate (si petit, sertis dans un grand mortier) | *mortier et pilon en agate (si petit, sertis dans un grand mortier) | ||
*proteinase K | |||
*cones avec filtres pour pipettes | |||
*tubes 1,5 ml microcentri en plastique sans RNase/DNase | |||
*tubes 15 ml style Falcon si grandes quantites | |||
==Procedure== | ==Procedure== | ||
