DEPC: Difference between revisions

DEPC (view source)

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Revision as of 11:12, 8 December 2006 (view source) Reshma P. Shetty (talk \| contribs) No edit summary		Revision as of 11:12, 8 December 2006 (view source) Reshma P. Shetty (talk \| contribs) No edit summary Newer edit →
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	DEPC is an acronym for di-ethyl-propyl carbonate. It is typically used to treat water to remove RNases ~~for use with RNA~~.		DEPC is an acronym for di-ethyl-propyl carbonate. It is typically used to treat water to remove RNases.

	DEPC reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses. Treatment involves adding DEPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DEPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DEPC).		DEPC reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses. Treatment involves adding DEPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DEPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DEPC).