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TOP10 chemically competent cells: Difference between revisions
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TOP10 chemically competent cells (view source)
Revision as of 11:16, 27 November 2006
, 27 November 2006→5x Ligation Adjustment Buffer
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* Glycerol 46.8% (468 ml/liter) | * Glycerol 46.8% (468 ml/liter) | ||
* pH adjustment with 2.3% of a 10% acetic acid solution (23 ml/liter) | * pH adjustment with 2.3% of a 10% acetic acid solution (23 ml/liter) | ||
* water to 1 liter | |||
* autoclave or sterile filter | |||
* Test pH adjustment by mixing 4 parts ligation buffer + 1 part 5x ligation adjustment buffer and checking pH to be 6.3 - 6.5 | * Test pH adjustment by mixing 4 parts ligation buffer + 1 part 5x ligation adjustment buffer and checking pH to be 6.3 - 6.5 | ||
==References== | ==References== | ||
