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Lidstrom:Autoinduction Media: Difference between revisions
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→How does oxygenation affect protein production?
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*** Note the OD is measured by NanoDrop; multiply the OD value by 10 to convert to 1cm cuvette measurements. | *** Note the OD is measured by NanoDrop; multiply the OD value by 10 to convert to 1cm cuvette measurements. | ||
*** This gel also shows a set of cultures where extra glycerol was added; this didn't impact OD or protein/cell. | *** This gel also shows a set of cultures where extra glycerol was added; this didn't impact OD or protein/cell. | ||
** [[image:150404_autoinduction_of_ACS_2P2F_with_different_culture_volumes--protein_gel. | ** [[image:150404_autoinduction_of_ACS_2P2F_with_different_culture_volumes--protein_gel.png|upright=2.0|thumb|center|High oxygen --> >3x higher protein/cell]] | ||
*** These are OD normalized loadings of total cell protein for comparison of protein/cell. | *** These are OD normalized loadings of total cell protein for comparison of protein/cell. | ||
