Lidstrom: SDS-PAGE: Difference between revisions

Lidstrom: SDS-PAGE (view source)

245 bytes removed , 29 March 2015

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 * Setup one spacer plate and one cover plate in each gel holder
 ** The cover plate goes on the side of the spacer plate with the spacers in order to create a small gap between the plates
-** Put on the black counter top to pinch closed.  Don't put it on the absorbent mat, as this slight unevenness will increase your gel leakage rate.  Janet had ~33% of gels leak until she started doing it on the black counter without padding; now she has ~5-10% of poured gels leak.
+** Make sure the bottom edges are flat after they are clamped down in the stand.
-* Put the gel holder into the casting stand
 * '''Make a fresh 10% (w/vol) APS solution'''.  Don't use an "old" solution; the gel won't polymerize.  10% w/v = 0.1 g/mL.
 ** 1 month old seems to be safe, but up to 3 months may be ok.  It depends on how old the stock bottle is.