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Lidstrom: SDS-PAGE: Difference between revisions
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→Pour the Resolving Gel
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=== '''Pour the Resolving Gel'''=== | === '''Pour the Resolving Gel'''=== | ||
* ''' [https://docs.google.com/spreadsheets/d/1obm1M4y7tFMy1yS_ZUhgZq3RHVG6XddrkUgcM93fgPY/edit#gid=1867778655 Recipe] ''': | * ''' [https://docs.google.com/spreadsheets/d/1obm1M4y7tFMy1yS_ZUhgZq3RHVG6XddrkUgcM93fgPY/edit#gid=1867778655 Recipe] ''': | ||
[[image:sample_PAGE_recipes--modular.png|thumb|center|Sample PAGE recipes. If you download '''[https://docs.google.com/spreadsheets/d/1obm1M4y7tFMy1yS_ZUhgZq3RHVG6XddrkUgcM93fgPY/edit#gid=1867778655 this sheet]''', you can change the acrylamide percentages and batch sizes and the recipes will auto-update.]] | |||
** Don't add APS/TEMED until ready to pour | ** Don't add APS/TEMED until ready to pour | ||
** '''The APS solution should be < 1 month old.''' Up to 2 months might be ok, but we don't have great data for this. | ** '''The APS solution should be < 1 month old.''' Up to 2 months might be ok, but we don't have great data for this. | ||
