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Lidstrom: SDS-PAGE: Difference between revisions
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→Gel Prep
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* '''Make a fresh 10% (w/vol) APS solution'''. Don't use an "old" solution; the gel won't polymerize. 10% w/v = 0.1 g/mL. | * '''Make a fresh 10% (w/vol) APS solution'''. Don't use an "old" solution; the gel won't polymerize. 10% w/v = 0.1 g/mL. | ||
** 1 month old seems to be safe, but up to 3 months may be ok. It depends on how old the stock bottle is. | ** 1 month old seems to be safe, but up to 3 months may be ok. It depends on how old the stock bottle is. | ||
*** If you want to do a test scale batch in an eppendorf tube, [https://docs.google.com/spreadsheets/d/1obm1M4y7tFMy1yS_ZUhgZq3RHVG6XddrkUgcM93fgPY/edit#gid=705290414 this scaled-down recipe] may help. You can mix the ingredients in an eppendorf and see how fast they polymerize, or whether polymerization fails all together. | |||
** Also, if the APS bottle's contents look wet more than powdery, you might not get polymerization at all. | ** Also, if the APS bottle's contents look wet more than powdery, you might not get polymerization at all. | ||
