TE: Difference between revisions

TE (view source)

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 ==Notes==
 *Use Tris base and adjust the pH to 8.0 using HCl.
-*TE buffer is often used to store DNA.  However, you should realize that the EDTA in TE can chelate the Mg<sup>2+</sup> ions necessary for many reactions such as restriction digests and PCR.  So when using DNA that was suspended in TE you should keep track of the amount of EDTA in the mix to make sure there is still enough Mg<sup>2+</sup> for your reaction to proceed successfully.
+*TE buffer is often used to store DNA.  The EDTA in TE chelates Mg<sup>2+</sup> ions necessary for most processes causing DNA degradation, and thus any residual DNA degradation activity will be suppressed by the presence of EDTA.  However, EDTA, for the same reason, also prevents restriction digests, PCR, and ligations.  So when using DNA that was suspended in TE you should keep track of the amount of EDTA in the mix to make sure there is still enough Mg<sup>2+</sup> for your reaction to proceed successfully.