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TOP10 chemically competent cells: Difference between revisions
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TOP10 chemically competent cells (view source)
Revision as of 23:00, 8 November 2006
, 8 November 2006→Measurement of competence
| Line 56: | Line 56: | ||
* Hold on ice 0.5 hours | * Hold on ice 0.5 hours | ||
* Heat shock 60 sec at 42C | * Heat shock 60 sec at 42C | ||
* Add 250 μl SOC | * Add 250 μl [[SOC]] | ||
* Incubate at 37 C for 1 hour in 2 ml centrifuge tubes rotated | * Incubate at 37 C for 1 hour in 2 ml centrifuge tubes rotated | ||
** using 2ml centrifuge tubes for transformation and regrowth works well because the small volumes flow well when rotated, increasing aeration. | ** using 2ml centrifuge tubes for transformation and regrowth works well because the small volumes flow well when rotated, increasing aeration. | ||
