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Sauer:bis-Tris SDS-PAGE, the very best: Difference between revisions
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Sauer:bis-Tris SDS-PAGE, the very best (view source)
Revision as of 11:45, 18 December 2013
, 18 December 2013→3.5X gel buffer
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Note: <u>bis</u>-Tris is Bis(2-hydroxyethyl) aminotris (hydroxymethyl) methane (e.g. Sigma catalog# B 7535). | Note: <u>bis</u>-Tris is Bis(2-hydroxyethyl) aminotris (hydroxymethyl) methane (e.g. Sigma catalog# B 7535). | ||
We have changes our recipe slightly to make it easier to make and use. Prepare 1.0 M bis-Tris as a "3X". Using thirds helps when making the gel solutions in graduate conical tubes. | |||
===sample buffer=== | ===sample buffer=== | ||
