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BISC209/F13: Lab7: Difference between revisions
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→Test for Antibiotic Production
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Identify how many potential antibiotic producers you might have. Definitely test any isolates that are likely to be ''Actinomycetes'', ''Myxobacteria'', or ''Bacillus''. It is wise to test all of your isolates since the soil is the main source of microbes that supply the world's antibiotics. It's possible that you might discover the next great antimicrobial drug and get very rich by selling the patent for your discovery to a drug company. Remember that the discovery of penicillin was completely accidental. <BR><BR> | Identify how many potential antibiotic producers you might have. Definitely test any isolates that are likely to be ''Actinomycetes'', ''Myxobacteria'', or ''Bacillus''. It is wise to test all of your isolates since the soil is the main source of microbes that supply the world's antibiotics. It's possible that you might discover the next great antimicrobial drug and get very rich by selling the patent for your discovery to a drug company. Remember that the discovery of penicillin was completely accidental. <BR><BR> | ||
Using aseptic technique dip a sterile swab into the concentration adjusted culture tube you made to match the turbidity of the McFarland 0.5 standard and make an inoculation (as shown below) down the middle of a plate of nutrient agar. <BR> Make | 1. Using aseptic technique dip a sterile swab into the concentration adjusted culture tube you made to match the turbidity of the McFarland 0.5 standard and make an inoculation (as shown below) down the middle of a plate of nutrient agar. <BR> | ||
2. Make additional plates exactly like the first for each isolate to be tested. Label them carefully and incubate the plates for ~1 week at RT.<BR><BR> | |||
[[Image:strep1a.jpg]]<BR> | [[Image:strep1a.jpg]]<BR> | ||
