BISC110/F13: Series 1 Lab 3 Tetrahymena Investigation: Difference between revisions

BISC110/F13: Series 1 Lab 3 Tetrahymena Investigation (view source)

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 #In a microcentrifuge tube, add 50 μL of 1% ink solution and then add 50 μL of live ''Tetrahymena''. Mix gently and record the time.
 #What is the effective concentration of the ink after the addition of ''Tetrahymena''?
-#Add 20 μL of the ''Tetrahymena pyriformis'' and ink solution that you made in step 1 to a glass slide. Add a cover slip and view using the microscope. If you need to review how to focus the microscrope, refer to the directions in Lab 2.  You may need to adjust the field diaphragm or to lower the condenser a bit to see the cellular structures clearly. Record your observations in your notebook.
+#Add 20 μL of the ''Tetrahymena pyriformis'' and ink solution that you made in step 1 to a glass slide. Add a cover slip and view using the microscope. If you need to review how to focus the microscrope, refer to the directions in Lab 2.  You may need to adjust the condenser aperture diaphragm or to lower the condenser a bit to see the cellular structures clearly. Record your observations in your notebook.
 #Do you see any ink particles inside the ''Tetrahymena''? Be sure to note the time as soon as you notice an ink filled phagocytic vacuole forming. Continue observing the ''Tetrahymena''. Watch the ''Tetrahymena'' for 15 minutes, noting how many vacuoles form and where those vacuoles are in the cell.  Do they move?  Are the vacuoles uniform in size?