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BISC209/F13: Lab2: Difference between revisions
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→Abundance Test 2: Enumeration of Community Soil Microorganisms by Direct Count of Microbial DNA Stained & Viewed by Fluorescence Microscopy
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'''COUNTING & CALCULATIONS:'''<BR> | '''COUNTING & CALCULATIONS:'''<BR> | ||
The area of each field of view at 1000X using the Fluorescent scope is 10487 μmeters<sup>2</sup>. The area is determined for the microscope used, in our case, a NIKON 80i fluorescent microscope. The diameter of the filterable section of the borosilicate apparatus is 17 mm (8500 μmeter radius). Therefore, the area is 2.28 X 10<sup>8</sup>μmeter<sup>2</sup>. Multiply the number of microorganisms counted on the photomicrograph by a factor of 2.17X10<sup>4</sup> (2.28 X 10<sup>8</sup>μm<sup>2</sup> divided by 1.0487 x 10<sup>4</sup>μmeters<sup>2</sup>) to determine the number of organisms found in 1mL of filtrate of extract. Then correct for the 1:100 dilution factor of filtrate which is the number of organisms in 1 gram of wet soil. Convert this to the number of organisms in the community in 1 gram of '''dry weight''' soil. <BR><BR> | The area of each field of view at 1000X using the Fluorescent scope is 10487 μmeters<sup>2</sup>. The area is determined for the microscope used, in our case, a NIKON 80i fluorescent microscope. The diameter of the filterable section of the borosilicate apparatus is 17 mm (8500 μmeter radius). Therefore, the area is 2.28 X 10<sup>8</sup> μmeter<sup>2</sup>. Multiply the number of microorganisms counted on the photomicrograph by a factor of 2.17X10<sup>4</sup> (2.28 X 10<sup>8</sup>μm<sup>2</sup> divided by 1.0487 x 10<sup>4</sup>μmeters<sup>2</sup>) to determine the number of organisms found in 1mL of filtrate of extract. Then correct for the 1:100 dilution factor of filtrate which is the number of organisms in 1 gram of wet soil. Convert this to the number of organisms in the community in 1 gram of '''dry weight''' soil. <BR><BR> | ||
After each person has collected their data, post the numbers to the class spread sheet and, of course enter all calculations in your lab notebook. Record these numbers in scientific notation (and as numbers with no decimals and with an amazing number of zeros). <BR> | After each person has collected their data, post the numbers to the class spread sheet and, of course enter all calculations in your lab notebook. Record these numbers in scientific notation (and as numbers with no decimals and with an amazing number of zeros). <BR> | ||
