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IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/29: Difference between revisions
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IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/29 (view source)
Revision as of 11:32, 22 August 2013
, 22 August 2013→Floor 2
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==Floor 2== | ==Floor 2== | ||
Restriction digest reactions were performed on the original J04450 original plasmid and the J04450-Nde1 bio brick using (i) EcoR1 and Pst1 and (ii) Nde1 and Xba1. After incubation samples were run on agarose gel. Transformation was done on the AntgP-Neo biobrick (ligated samples 2b and 2c). Also, colonies 1 and 2 (used in colony PCR) from samples 1 and 2 were inoculated and grown overnight. | Restriction digest reactions were performed on the original J04450 original plasmid and the J04450-Nde1 bio brick using (i) EcoR1 and Pst1 and (ii) Nde1 and Xba1. After incubation samples were run on agarose gel ''fig 5''. Transformation was done on the AntgP-Neo biobrick (ligated samples 2b and 2c). Also, colonies 1 and 2 (used in colony PCR) from samples 1 and 2 were inoculated and grown overnight. | ||
[[Image:29 july.JPG|thumb| Restriction digest of J04450 original plasmid (lanes 1 and 3) and J04450-Nde1 biobrick (lanes 2 and 4) using i) EcoR1 and Pst1 and (ii) Nde1 and Xba1.]] | [[Image:29 july.JPG|thumb|Fig 5:Restriction digest of J04450 original plasmid (lanes 1 and 3) and J04450-Nde1 biobrick (lanes 2 and 4) using i) EcoR1 and Pst1 and (ii) Nde1 and Xba1.]] | ||
