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| If you have time, you can try to confirm a positive preliminary motility test by doing a hanging drop motility wet mount or a flagella stain. See the Protocols section in the wiki on [[BISC209/F13: Motility | Motility Tests]] for directions on performing confirmation tests.<BR><BR> | | If you have time, you can try to confirm a positive preliminary motility test by doing a hanging drop motility wet mount or a flagella stain. See the Protocols section in the wiki on [[BISC209/F13: Motility | Motility Tests]] for directions on performing confirmation tests.<BR><BR> |
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| ==Continue Antibiotic Production test started last week==
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| '''Week 2'''<BR>
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| Need fresh control cultures of ''Eschericia coli'' (Gram negative), ''Staphylococcus epidermidis'' (Gram positive) and ''Micrococcus luteus'' (Gram positive) grown in nutrient broth to the same turbidity standard used last week for isolate cultures. Use the MacFarland Standard to make sure that these cultures are of the same turbidity as your isolates were when you applied them to the NA plates last week. If not, dilute a little of the stock control cultures (in separate sterile tubes)to appropriate turbidity with nutrient broth.<BR>
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| '''PROTOCOL'''<BR><BR>
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| '''Use the cultures of your isolates set up last week on NA.<BR>
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| Use a sterile swab to aseptically apply parallel lines of inoculation of each of the control broth cultures of : ''E. coli'', ''Micrococcus'', and ''S. epidermidis'' as shown in the illustration below. Use a different sterile swab for each culture. These parallel inoculation lines should be made perpendicular to the putative antibiotic producer's (''your isolate's'') colony growth. (See the illustration.) Be careful not to touch the putative antibiotic producer's growth with the control cultures, but come as close as you can. Make a template in your lab notebook and label the plate to indicate where each control culture is streaked. Incubate these culture plates at RT in a place that where your instructor can monitor their development. One person/lab should also set up viability controls by swabbing each the three broth cultures onto separate areas of another NA plate. If you see growth of each of the controls next week, we will be sure that any inhibition of growth is due to sensitivity to a diffused antibiotic rather than lack of growth occurring because one or more of the control broth cultures you used today lacked enough viable cells to form colonies on your test plate.<BR>
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| [[Image:Antibiotic2.jpg]]
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| <BR><BR>
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| ==Antagonistic and Mutualistic Interactions Analysis== | | ==Antagonistic and Mutualistic Interactions Analysis== |
