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==Floor 2== | ==Floor 2== | ||
Restriction digest reactions were performed on the original J04450 original plasmid and the J04450-Nde1 bio brick using (i) EcoR1 and Pst1 and (ii) Nde1 and Xba1. After incubation samples were run on agarose gel. Transformation was done on the AntgP-Neo biobrick (ligated samples 2b and 2c). Also, colonies 1 and 2 (used in colony PCR) from samples 1 and 2 were inoculated and grown overnight. | |||
[[Image:29 july.JPG|thumb|Fig. ]] | [[Image:29 july.JPG|thumb|Fig. ]] |
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