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BISC220/S14: Mod 1 Lab 5: Difference between revisions
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→Enzyme Kinetics
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== Enzyme Kinetics == | == Enzyme Kinetics == | ||
In this laboratory, the effect of substrate concentration on the reaction velocity of β-galactosidase will be measured in the presence and absence of an inhibitor. You will also determine the kinetic parameters V<sub>max</sub>, Km, and Ki for the reactions. To conduct these experiments you will use the partially purified β-galactosidase fraction obtained from the affinity chromatography protocol in Lab | In this laboratory, the effect of substrate concentration on the reaction velocity of β-galactosidase will be measured in the presence and absence of an inhibitor. You will also determine the kinetic parameters V<sub>max</sub>, Km, and Ki for the reactions. To conduct these experiments you will use the partially purified β-galactosidase fraction obtained from the affinity chromatography protocol in Lab 3. <br> | ||
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'''Enzyme Kinetics'''<br> | '''Enzyme Kinetics'''<br> | ||
