2,695
edits
Lidstrom:Ultracentrifuge: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Tubes collapsing
(→Basics) |
|||
| Line 14: | Line 14: | ||
** See below if you want to buy tubes for smaller volumes. | ** See below if you want to buy tubes for smaller volumes. | ||
** You probably won't be able to get any sample out of a collapsed tube. | ** You probably won't be able to get any sample out of a collapsed tube. | ||
** When they collapse it is very tricky to get them out. '''Consult the rotor manual if you get tubes stuck.''' They recommend autoclaving the | ** When they collapse it is very tricky to get them out. '''Consult the rotor manual if you get tubes stuck.''' They recommend autoclaving the buckets upside down instead of trying to fish/pry the tubes out. | ||
=== How balanced does it need to be? === | === How balanced does it need to be? === | ||
* Tech support 5/2013 [[User:Janet B. Matsen|JM]] said that you can balance oppositely placed buckets by eye. There is no need to spend the time weighing down to the nearest milligram. The centrifuge will stop itself if it is off balance. The auto shut-off feature is conservative enough that you cannot hurt the rotor by having too imbalanced of samples. You can, however, waste your time if you need to rebalance and restart the ultracentrifuge. | * Tech support 5/2013 [[User:Janet B. Matsen|JM]] said that you can balance oppositely placed buckets by eye. There is no need to spend the time weighing down to the nearest milligram. The centrifuge will stop itself if it is off balance. The auto shut-off feature is conservative enough that you cannot hurt the rotor by having too imbalanced of samples. You can, however, waste your time if you need to rebalance and restart the ultracentrifuge. | ||
