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Lidstrom:Ultracentrifuge: Difference between revisions
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== Basics == | == Basics == | ||
* If you desire 4<sup>o</sup>C during your spin cycle, you must allow the machine to cool to that point before it will allow you to press start. | * If you desire 4<sup>o</sup>C during your spin cycle, you must allow the machine to cool to that point before it will allow you to press start. | ||
=== Tubes collapsing=== | |||
* '''If you use too small of a volume (< half full) in the ultracentrifuge tubes, they will collapse!''' | * '''If you use too small of a volume (< half full) in the ultracentrifuge tubes, they will collapse!''' | ||
** [[image:tubes collapsed in ultracentrifuge.jpg|thumb|center|Tubes with only 1/2 mL liquid collapse in ultracentrifuge.]] | ** [[image:tubes collapsed in ultracentrifuge.jpg|thumb|center|Tubes with only 1/2 mL liquid collapse in ultracentrifuge.]] | ||
** | ** 1/2 mL '''definitely''' collapsed on [[User:Janet B. Matsen|Janet]] in 5/2013 before I called tech support and learned the golden "1/2 full rule." | ||
** | ** See below if you want to buy tubes for smaller volumes. | ||
** You probably won't be able to get any sample out of a collapsed tube. | ** You probably won't be able to get any sample out of a collapsed tube. | ||
** When they collapse it is very tricky to get them out. | ** When they collapse it is very tricky to get them out. '''Consult the rotor manual if you get tubes stuck.''' They recommend autoclaving the rotor upside down instead of trying to fish/pry the tubes out. | ||
=== How balanced does it need to be? === | === How balanced does it need to be? === | ||
* Tech support 5/2013 [[User:Janet B. Matsen|JM]] said that you can balance oppositely placed buckets by eye. There is no need to spend the time weighing down to the nearest milligram. The centrifuge will stop itself if it is off balance. The auto shut-off feature is conservative enough that you cannot hurt the rotor by having too imbalanced of samples. You can, however, waste your time if you need to rebalance and restart the ultracentrifuge. | * Tech support 5/2013 [[User:Janet B. Matsen|JM]] said that you can balance oppositely placed buckets by eye. There is no need to spend the time weighing down to the nearest milligram. The centrifuge will stop itself if it is off balance. The auto shut-off feature is conservative enough that you cannot hurt the rotor by having too imbalanced of samples. You can, however, waste your time if you need to rebalance and restart the ultracentrifuge. | ||
=== When it wont' try to start === | |||
* If the ultracentrifuge won't start and won't even try to start, open the door, lift the rotor up, place it back down, let it cool back down to your desired temp, and hold start. | |||
== Example Protocols == | == Example Protocols == | ||
