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** [[image:tubes collapsed in ultracentrifuge.jpg|thumb|center|Tubes with only 1/2 mL liquid collapse in ultracentrifuge.]] | ** [[image:tubes collapsed in ultracentrifuge.jpg|thumb|center|Tubes with only 1/2 mL liquid collapse in ultracentrifuge.]] | ||
** It is hard to say how much is enough, but it seems to be about 1 mL. 1/2 mL '''definitely''' collapsed on [[User:Janet B. Matsen|Janet]] in 5/2013. | ** It is hard to say how much is enough, but it seems to be about 1 mL. 1/2 mL '''definitely''' collapsed on [[User:Janet B. Matsen|Janet]] in 5/2013. | ||
** We should be able to find smaller-volume tubes for our rotor. ([https://www.beckmancoulter.com/wsrportal/wsr/research-and-discovery/products-and-services/centrifugation/optima-max-xp/index.htm link]) | |||
** You probably won't be able to get any sample out of a collapsed tube. | ** You probably won't be able to get any sample out of a collapsed tube. | ||
** When they collapse it is very tricky to get them out. Needle nose pliers are probably your best bet but it can still be very hard. | ** When they collapse it is very tricky to get them out. Needle nose pliers are probably your best bet but it can still be very hard. |
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