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Lidstrom:Ultracentrifuge: Difference between revisions
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(New page: Back to Protocols == Basics == * If you desire 4<sup>o</sup>C during your spin cycle, you must allow the machine to cool to that point before it will allow you to ...) |
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== Example Protocols == | == Example Protocols == | ||
* To spin down the membranes and membrane-bound proteins in a crude extract, centrifuge for | * To spin down the membranes and membrane-bound proteins in a crude extract, centrifuge for 45,000 RPM at 4<sup>o</sup>C for 1 hour. With our MLS-50 mushroom-shaped rotor, this is 163,000xg at r<sub>average</sub> (108,000xg at r<sub>minimum</sub>). | ||
