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TE: Difference between revisions
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TE is a shorter name for "Tris-EDTA". For the rest of the story, see [[TAE]], [[Tris]], and [[TBE]]. | |||
==Purpose== | ==Purpose== | ||
TE buffer is often used to store DNA and RNA. | * TE buffer is often used to store DNA and RNA. | ||
* [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes. | * [[EDTA]] in TE chelates Mg<sup>2+</sup> and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes. | ||
* [[Tris]] is a buffering agent to keep the solution at a defined pH. | * [[Tris]] is a buffering agent to keep the solution at a defined pH. | ||
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* [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8018 10x TE buffer at Cold Spring Harbor Protocols] | * [http://cshprotocols.cshlp.org/cgi/content/full/2006/3/pdb.rec8018 10x TE buffer at Cold Spring Harbor Protocols] | ||
* [http://www.cytographica.com/lab/solutions/TE.htm 10x and 1x TE at cytographica] | * [http://www.cytographica.com/lab/solutions/TE.htm 10x and 1x TE at cytographica] | ||
* http://en.wikipedia.org/wiki/TE_buffer | |||
[[Category:Material]] | [[Category:Material]] | ||
[[Category:Buffers]] | [[Category:Buffers]] | ||
