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BISC110/S13: Series 1 Lab 1 Boot Camp: Difference between revisions
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BISC110/S13: Series 1 Lab 1 Boot Camp (view source)
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#Use a clean pipette tip to add 100 μL of 1/8 dilution to the tube labeled 1/16; mix. | #Use a clean pipette tip to add 100 μL of 1/8 dilution to the tube labeled 1/16; mix. | ||
#Using a clean pipette tip, remove 100 μL of solution from the 1/16 tube, and discard it in the sink. You now have four tubes containing 100 μL of four different working concentrations of your reagent. Observe the color intensity of the four solutions and record in your lab notebook. Also, observe the relative volumes of the four solutions. If they are different from each other, then inaccurate pipetting has occurred. Save these dilutions for use next week.<BR> | #Using a clean pipette tip, remove 100 μL of solution from the 1/16 tube, and discard it in the sink. You now have four tubes containing 100 μL of four different working concentrations of your reagent. Observe the color intensity of the four solutions and record in your lab notebook. Also, observe the relative volumes of the four solutions. If they are different from each other, then inaccurate pipetting has occurred. Save these dilutions for use next week.<BR> | ||
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=='''Assignments'''== | |||
#Familiarize yourself with the Lab wiki and bookmark it on your computer. Read material relating to course assignments and lab attendance, as well as instructions about lab notebooks in the [[BISC110/S13 | '''Introduction To Cell Biology''' ]] page and in the [[BISC110/S13:Resources | '''Resources''']] section. Be sure to familiarize yourself with the safety information (also found in the [[BISC110/S13:Resources | '''Resources''']] section). | |||
#Before you come to lab next time, read all the material in Lab 2 and outline the day's work in your lab notebook according to the example your instructor went over for lab 1. | |||
#Make sure that you understand all the concepts covered in Lab 1. Solve the practice problems below on a piece of paper (NOT IN YOUR LAB NOTEBOOK!) and hand them in at the beginning of lab 2. The practice problems must be done individually. Your instructor will grade the problems and return them to you in lab 3. | |||
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'''Practice Problems (5 points)''' | |||
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[[Media:Lab_1_Practice_Problems110.doc]] Download Assignment below in Word format through this link<br> | |||
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In the following problems, please show all calculations, including the units. To receive full credit, make sure to answer all parts of each question. | |||
#a) How many grams of sucrose (MW=342) would you need to make 100mL of a 10<sup>-2</sup> M sucrose solution? b) How many milligrams of sucrose would you need to make this same solution? c) Express 10<sup>-2</sup> M as a millimolar (mM) concentration. | |||
#Compound Z has a MW of 100. Your lab partner weighed 25 grams of compound Z and dissolved it in water to a final volume of 1 liter. a) What is the concentration of the solution expressed as a percentage by weight (w/v)? b) What is the concentration of the solution expressed as molarity? | |||
#Convert a 0.26M solution of cobalt chloride hexahydrate (MW=237.9) into %(w/v) units. b) How would you make a 5% (v/v) solution of ethanol from 100% ethanol? | |||
#You need to prepare 2 methanol solutions for lab today: a) 300mL of 50% (v/v) methanol and b) 200mL of 25% (v/v) methanol. You have been supplied with 100% methanol and deionized water as well as graduated cylinders and beakers of the appropriate sizes, a magnetic stirrer and Teflon stir bars. How would you use these materials to prepare the two methanol solutions? Note that this question requires more than just showing the math involved. You need to write the steps involved in preparing the solutions, including when and how you use the provided materials. | |||
#Practice converting between the following units by writing your answers in decimal form and in scientific notation: [[Image:BISC110.1.3.jpg]] | |||
#For each of the following situations, please describe what you would do. <br> | |||
a. You just spilled Tetrahymena on your arm. <br> | |||
b. You just finished pipetting some water with a 10ml serologic pipet. Where do you put the pipet?<br><BR> | |||
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=='''Links To Other Labs in this Series'''== | |||
