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BME103:T930 Group 16 l2: Difference between revisions
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2. Label remaining pipettes and tubes to correlate with the samples, and label another tube and pipette for the provided Calf-Thymus (which acts as another positive control.) One pipette should be set aside for the transfer of SYBR Green and another should be labeled for waste. | 2. Label remaining pipettes and tubes to correlate with the samples, and label another tube and pipette for the provided Calf-Thymus (which acts as another positive control.) One pipette should be set aside for the transfer of SYBR Green and another should be labeled for waste. | ||
3. | 3. Once everything is labeled, transfer the DNA samples using the specifically labeled pipette (to avoid cross contamination do not use a pipette that has touched a DNA sample to transfer a different sample). The DNA should be released into an eppendorf tube containing 400 mL of buffer. Repeat this procedure for all samples and controls including the Calf Thymus. | ||
4. On the rough side of the provided glass slides place two drops of SYBR Green solution with the SYBR green marked pipette over two of the holes in the slide. | |||
5. Place two drops of one of your sample (using that samples specifically labeled pipette) on top of the SYBR green. | |||
6. Carefully move the slide in place so that the light is on it. | |||
7. Place the slide and phone under the black box and take a picture. | |||
8. Using the pipette marked for 'waste,' remove the solution from the slide. | |||
9. Repeat steps four through 8 for all DNA samples and controls including water. | |||
==Research and Development== | ==Research and Development== | ||
