32
edits
BME103:T930 Group 16 l2: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Protocols
| Line 77: | Line 77: | ||
|- | |- | ||
| Calf Thymus DNA|| 50.0 mL | | Calf Thymus DNA|| 50.0 mL | ||
|- | |||
| Measurement Buffer|| 400 mL | |||
|- | |- | ||
| Dark Room (Black Box) || 1 | | Dark Room (Black Box) || 1 | ||
| Line 128: | Line 130: | ||
'''DNA Measurement Protocol''' | '''DNA Measurement Protocol''' | ||
1. When the PCR has finished replicating the DNA samples, remove the tubes from the machine. | |||
2. Label remaining pipettes and tubes to correlate with the samples, and label another tube and pipette for the provided Calf-Thymus (which acts as another positive control.) One pipette should be set aside for the transfer of SYBR Green and another should be labeled for waste. | |||
3. | |||
==Research and Development== | ==Research and Development== | ||
