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BME103:T930 Group 16 l2: Difference between revisions
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Before starting the experiment be sure that the solar panels on the PCR machine have been charged. | Before starting the experiment be sure that the solar panels on the PCR machine have been charged. | ||
To prepare the DNA samples for the PCR, label the DNA test tubes with the patients name and replication number or label it in some way so that you will be able to distinguish between the patient sample and which replication it is (for example P1 R1 for patient one replication one). After you have labeled all of your patient samples and your positive and negative control, you are ready to add the PCR reaction mix. | To prepare the DNA samples for the PCR, label the DNA test tubes with the patients name and replication number or label it in some way so that you will be able to distinguish between the patient sample and which replication it is (for example P1 R1 for patient one replication one). After you have labeled all of your patient samples and your positive and negative control, you are ready to add the PCR reaction mix. The reaction mix includes: Taq DNA polymerase, forward primer, reverse primer, MgCl2 and dNTP's. | ||
