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BISC110/F12: Series 3 Experiment 9 Hill Reaction: Difference between revisions
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BISC110/F12: Series 3 Experiment 9 Hill Reaction (view source)
Revision as of 19:27, 8 November 2012
, 8 November 2012→Procedure
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#If the majority of your data points are within the linear portion of the curve repeat steps 2-6. Your goal is to complete 3 runs that give approximately the same rate. <BR> | #If the majority of your data points are within the linear portion of the curve repeat steps 2-6. Your goal is to complete 3 runs that give approximately the same rate. <BR> | ||
If your rate is too slow then increase the light intensity. <BR> | If your rate is too slow then increase the light intensity. <BR><BR> | ||
If your rate is too fast then decrease the light intensity or decrease the amount of thylakoids. To decrease the amount of thylakoids use 50 µL of a ½ dilution of the thylakoid suspension (use resuspension buffer for dilution) OR use 25 µl of thylakoids for both your blank and reaction. | If your rate is too fast then decrease the light intensity or decrease the amount of thylakoids. To decrease the amount of thylakoids use 50 µL of a ½ dilution of the thylakoid suspension (use resuspension buffer for dilution) OR use 25 µl of thylakoids for both your blank and reaction. | ||
<BR> | <BR> | ||
