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Knight:Annealing and primer extension with Klenow polymerase: Difference between revisions
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Knight:Annealing and primer extension with Klenow polymerase (view source)
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==Materials== | ==Materials== | ||
*Two oligos which overlap by ~20 bp and have restriction enzyme sites at the 5' ends as in the diagram below. See [[Restriction Digest]] for | *Two oligos which overlap by ~20 bp and have restriction enzyme sites at the 5' ends as in the diagram below. See [[Restriction Digest#Notes | restriction digest notes]] for information on cutting near the ends of linear DNA fragments. See [[Annealing and primer extension#Notes | notes]] for more information on primer ordering. | ||
Oligo 1: 5' ---RE site-------------------------------- 3'<br> | Oligo 1: 5' ---RE site-------------------------------- 3'<br> | ||
