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IGEM:Paris Bettencourt 2012/Previous Biosafety iGEM Projects: Difference between revisions
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IGEM:Paris Bettencourt 2012/Previous Biosafety iGEM Projects (view source)
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#: Within 2 hours after addition of IPTG, the OD660 went down by 80%, indicating that the lysis genes were successfully induced by IPTG. However, we should wonder if 80% is good enough... | #: Within 2 hours after addition of IPTG, the OD660 went down by 80%, indicating that the lysis genes were successfully induced by IPTG. However, we should wonder if 80% is good enough... | ||
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| <center> Duke </center> | |||
| <center> 2009 </center> | |||
| <center> [http://2009.igem.org/Team:Illinois-Tools IMPtools] </center> | |||
| "Interactive Metabolic Pathway Tools (IMP Tools) is an open source, web based program that involves model-guided cellular engineering where new metabolic functions can be added to existing microorganisms. | |||
*This program will assist in the design stage of synthetic biology research. | |||
* It takes a user-defined input compound, output compound, and weighting scheme and determines the ideal pathway from the starting to the ending compound." | |||
| "ATP is the energy within a cell and a majority of what it relies on to flourish. """"""By limiting ATP produced we lessen the chances of a cell growing vigorously and being a potential danger to the environment"""""". | |||
*The actual means to prevent this is by creating a script that will analyze ATP consumption and production. | |||
* With this information, you are able to adjust the ATP metabolism in whatever way you want. | |||
*You will be able to keep the production low enough so cell processes can still occur and allow the cell to grow to an extent but not high enough that it can potentially grow out of control. " | |||
<p style="text-align:right;"> [http://2009.igem.org/Team:Illinois-Tools/Safety Read More] </p> | |||
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| <center> UCSF </center> | |||
| <center> 2010 </center> | |||
| <center> [http://2010.igem.org/Team:UCSF Synthetic Cancer Killers] </center> | |||
| "Killer cells of the immune system identify cancer and pathogen-infected cells and kill them. These potent killers travel throughout the body, recognizing proteins and other molecules on the surface of cells. In order to differentiate between healthy and diseased cells, killer cells use a variety of receptors, which bind to specific ligands on the target cells’ surface. If the target cell is deemed potentially dangerous, the killer cell grips the target cell tightly and creates an immunological synapse at the site of adhesion. Within this immunological synapse, the killer cell releases cytotoxic granules to kill the target cell without harming nearby cells, triggering a directed apoptotic response. | |||
Our team will focus on improving killer cells’ specificity and killing efficiency towards cancerous target cells. By using tools of synthetic biology, we hope to create powerful killing bio-machines to fight cancer. Our newly engineered synthetic devices would have the potential to enhance current adoptive cell-based immunotherapy for cancer patients." | |||
| We believe that as long as the proper precautions are taken and the safety guidelines are followed, most potential safety concerns can be prevented. None of our genes, parts, or devices are considered potentially oncogenic or pathogenic which would require a safety rating above BSL1. We specifically chose not to use materials from known pathogens. A possible extra precaution to make parts, devices, and systems safer would have been to put suicide genes into the sequences to prevent unintended introductions of them into the environment, but this was less necessary based upon the components and systems we used. | |||
<p style="text-align:right;"> [http://2010.igem.org/Team:UCSF/Safety Read More] </p> | |||
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| <center> University of Waterloo </center> | |||
| <center> 2008 </center> | |||
| <center> [http://2008.igem.org/Team:Waterloo Genome-free Bacterial Bioproduct Factory: ] </center> | |||
| A bacterial cell already containing a plasmid encoding bioproduct synthesis genes, will self-destruct by degrading its own genome and transiently produce the bioproduct until cell resources have been exhausted. | |||
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<p style="text-align:right;"> [http://2008.igem.org/Team:Waterloo/Project Read More] </p> | |||
| No result, bronze medal | |||
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| <center> Bielefeld-Germany </center> | |||
| <center> 2011 </center> | |||
| <center> [http://2011.igem.org/Team:Bielefeld-Germany the Bisphenol-A team] </center> | |||
| "The development of sensitive and selective biosensors is an important research field in synthetic biology. Biosensors can be applied in a wide range of uses - from the detection of environmental toxics up to clinical diagnostics. Because cells have to sense their surroundings, there are a lot of natural systems that are similar to a biosensor. Prejudicial cellular biosensors often show negative side effects that complicate any practical application. Common problems are the limited use outside of a gene laboratory due to the use of genetically engineered cells, the low durability because of the usage of living cells and the appearance of undesired signals induced by endogenous metabolic pathways. | |||
To solve these problems, the iGEM-Team Bielefeld 2011 aims to develope a cell-free bisphenol A (BPA) biosensor based on a coupled enzyme reaction fused to S-layer proteins for everyday use. Bisphenol A is a supposedly harmful substance which is used in the production of polycarbonate. To detect BPA it is degraded by a fusion protein under formation of NAD+ which is detected by an NAD+-dependent enzymatic reaction with a molecular beacon. Both enzymes are fused to S-layer proteins which build up well-defined nanosurfaces and are attached to the surface of beads. By providing these nanobiotechnological building blocks the system is expandable to other applications." | |||
| Our approach is a cell free biosensor. One advantage is that no living organisms need to be used outside the lab for the application of our system. We want to provide S-layers as nanobiotechnological building blocks for cell free biosensors. By fusing different enzymes to these proteins, a variety of biosensors can be build. Further cell free applications are possible and we think that when more projects are focusing on cell free systems this is also a contribution to more safety and security. All GMOs can stay in the lab, therefore are grown under controlled conditions and only qualified personnel has access to them. | |||
<p style="text-align:right;"> [http://2011.igem.org/Team:Bielefeld-Germany/Safety Read More] </p> | |||
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| <center> CHIBA </center> | |||
| <center> 2010 </center> | |||
| <center> [http://2010.igem.org/Team:Chiba Double Click] </center> | |||
| We’re inspired by double-click of computer’s mouse. It doesn’t react to the first click but does react when it is accompanied by the second one. This is one of the most accepted, familiarized, and proven mechanism to diminish the erroneous operation. This fail-safe technology should find various uses also in biotechnology. | |||
| "If there is only an input, nothing happens. | |||
Duration of the input is not the matter. The circuit cares only the number of input. | |||
However, a certain time after the 1st input, it returns to the initial state. | |||
Giving two inputs in the limited time the circuit get activated (gives output)." | |||
<p style="text-align:right;"> [http://2010.igem.org/Team:Chiba/Safety Read More] </p> | |||
| System did not work | |||
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| <center> HKU-Hong Kong </center> | |||
| <center> 2010 </center> | |||
| <center> [http://2010.igem.org/Team:HKU-Hong_Kong The Bio-Safety Net] </center> | |||
| "By using different promoters, the system can respond to changes in environmental factors and control expression specific to a chosen factor. Such mechanism can be easily assembled and incorporated into bacteria through the use of biobricks. | |||
Our team’s project is a “bio-safety net” that limits the survival of bacteria according to tailored conditions." | |||
| Killswitch using T4 holin ,T4 anti holin, and lysozyme system (or Gene E), under control of pBAD and pLAC | |||
<p style="text-align:right;"> [http://2010.igem.org/Team:HKU-Hong_Kong/Safety Read More] </p> | |||
| After 8 hours, cells implementing this circuit had an OD of 0.0309 versus an OD of 1.9414 and 1.9651 in cells where the circuit was suppressed. | |||
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