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IGEM:Paris Bettencourt 2012/Previous Biosafety iGEM Projects: Difference between revisions
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IGEM:Paris Bettencourt 2012/Previous Biosafety iGEM Projects (view source)
Revision as of 17:16, 11 September 2012
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*Another similar “suicide” containment system uses streptavidin (BBa_J36841) (Kaplan, Mello et al. 1999; Urgun-Demirtas, Stark et al. 2006). This protein binds very tightly to biotin, a required co-enzyme for many metabolic pathways. This makes biotin unavailable and causes cell death. Kaplan et al. reported cell counts were reduced 99.9% in eight hours after their system was activated by absence of pollutant to degrade. | *Another similar “suicide” containment system uses streptavidin (BBa_J36841) (Kaplan, Mello et al. 1999; Urgun-Demirtas, Stark et al. 2006). This protein binds very tightly to biotin, a required co-enzyme for many metabolic pathways. This makes biotin unavailable and causes cell death. Kaplan et al. reported cell counts were reduced 99.9% in eight hours after their system was activated by absence of pollutant to degrade. | ||
<p style="text-align:right;"> [http://2011.igem.org/Team:Caltech/Biosafety Read More] </p> | <p style="text-align:right;"> [http://2011.igem.org/Team:Caltech/Biosafety Read More] </p> | ||
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| <center> Berkeley</center> | | <center> Berkeley</center> | ||
| <center> 2010 </center> | | <center> 2010 </center> | ||
| <center> [http:// | | <center> [http://2010.igem.org/Team:Berkeley] </center> | ||
| Choa Choa's Delivery Service | | Choa Choa's Delivery Service | ||
| Clotho Framework: | | Clotho Framework: | ||
Clotho implements the current biosafety standards as outlined by the NIH and the CDC. Whenever a new part is instantiated, Clotho BLAST's its sequence against a databank of known virulence factors and pathogens and returns the RG number of the highest match. This framework ensures that every part in Clotho has a RG value associated with it. In addition, when composite parts are made by joining basic parts, the composite part is also assigned a BSL number. Finally, all strains in Clotho have a risk group. | Clotho implements the current biosafety standards as outlined by the NIH and the CDC. Whenever a new part is instantiated, Clotho BLAST's its sequence against a databank of known virulence factors and pathogens and returns the RG number of the highest match. This framework ensures that every part in Clotho has a RG value associated with it. In addition, when composite parts are made by joining basic parts, the composite part is also assigned a BSL number. Finally, all strains in Clotho have a risk group. | ||
<p style="text-align:right;"> [http:// | <p style="text-align:right;"> [http://2010.igem.org/Team:Berkeley/Clotho Read More] </p> | ||
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| Promoter responsive to synthetic chemicals not present in nature. | | Promoter responsive to synthetic chemicals not present in nature. | ||
<p style="text-align:right;"> [http://2011.igem.org/Team:WITS-CSIR_SA/Project/Safety Read More] </p> | <p style="text-align:right;"> [http://2011.igem.org/Team:WITS-CSIR_SA/Project/Safety Read More] </p> | ||
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| FLP recombination to remove resistance gene, eg. FRT - Cm - FRT will remove the Cm resistance gene | | FLP recombination to remove resistance gene, eg. FRT - Cm - FRT will remove the Cm resistance gene | ||
<p style="text-align:right;"> [http://2011.igem.org/Team:ULB-Brussels/removing Read More] </p> | <p style="text-align:right;"> [http://2011.igem.org/Team:ULB-Brussels/removing Read More] </p> | ||
| "All 32 candidates that contained pINDEL grew on LB but none of them grew on LB Cm plates indicating that all of them have lost the antibiotic resistance cassette, sgowing the the DEL function of pINDEL functions properly." | |||
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