Making a long term stock of bacteria: Difference between revisions

Making a long term stock of bacteria (view source)

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 ==Method==
-*Add 1 ml of 40% glycerol in H<sub>2</sub>O to a cryogenic vial.
+#Pick a single colony of the clone off a plate and grow an overnight in the appropriate selectable liquid medium (3-5ml).
-*Add 1 ml sample from the culture of bacteria to be stored.
+#Add 0.5 ml of 40% glycerol in H<sub>2</sub>O to a cryogenic vial.
-*Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed.
+#Add 0,5 ml sample from the culture of bacteria to be stored.
-**Alternatively, pipet to mix.
+#Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed.
-*Use a tough spot to put the name of the strain or some useful identifier on the top of the vial.
+#*Alternatively, pipet to mix.
-*On the side of the vial list all relevant information - part, vector, strain, date, researcher, etc.
+#On the side of the vial list all relevant information - part, vector, strain, date, researcher, etc.
-*Store in a freezer box in a -80C freezer.  Remember to record where the vial is stored for fast retrieval later.
+#Freeze glycerol stock in liquid nitrogen and store in a -80C freezer.
+#*This will also be a good time to record the strain information and record the location.
 ==Notes==