- 40% glycerol solution
- Cryogenic vials
- Add 1 ml of 40% glycerol in H2O to a cryogenic vial.
- Add 1 ml sample from the culture of bacteria to be stored.
- Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed.
- Alternatively, pipet to mix.
- Use a tough spot to put the name of the strain or some useful identifier on the top of the vial.
- On the side of the vial list all relevant information - part, vector, strain, date, researcher, etc.
- Store in a freezer box in a -80C freezer. Remember to record where the vial is stored for fast retrieval later.
- While it is possible to make a long term stock from cells in stationary phase, ideally your culture should be in logarithmic growth phase.