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==Pad Preparation== | ==Pad Preparation== | ||
1. Microwave 2% agarose (mix of low-melt and normal, to taste) in Thorn media (see below). | 1. Microwave 2% agarose (mix of low-melt and normal, to taste) in Thorn media (see below). (If you have used different percentages of agarose pads please let us know, bruno) | ||
2. Apply 2mL to a 24x60 mm coverslip. | 2. Apply 2mL to a 24x60 mm coverslip. |
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