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IGEM:Virginia 2012/Protocols/PEG/NaCl Preparation: Difference between revisions
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IGEM:Virginia 2012/Protocols/PEG/NaCl Preparation (view source)
Revision as of 10:38, 30 May 2012
, 30 May 2012→Procedure
(New page: ==Materials== *20% PEG-8000 *2.5 M NaCl *milliQ H2O *1 L bottle *Stir bar ==Procedure== # Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to st...) |
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# Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl. | # Fill a 1 L bottle with 400 mL of milliQ H2O and put in a stir bar. Use the magnetic mixer to stir while you add the PEG and NaCl. | ||
# Measure out 80g of PEG-8000 and add it slowly (it might be easier to add 20g | # Measure out 80g of PEG-8000 and add it slowly (it might be easier to add 20g four times or 40g twice). | ||
# Measure 58.4g NaCl and add it slowly. | # Measure 58.4g NaCl and add it slowly. | ||
